The method of conjugation is a critical element in the development and manufacture of any lateral flow assay. The potential conjugation technologies have grown over recent years as the demand for quantitative and more sensitive assays has increased. In this blog, we explore the options open to you. Whilst latex continues to be used effectively in a number of lateral flow assays, innovation has led to a range of alternative options being available including gold, silver, platinum, carbon, fluorescence and magnetic.
Latex beads can be used in a wide range of applications, but are especially popular for lateral flow assays and latex agglutination experiments. Lateral flow assays LFA are used to quickly and easily establish whether a target analyte is present in a test sample. In a typical LFA, the sample is added to a sample application pad at one end of a test strip, from which it flows to a conjugate release pad, into which the detection reagent has been dried. The detection reagent is commonly an antibody which has been conjugated to a colored moiety such as a latex bead.
Our Latex Conjugation Kit allows antibodies or proteins to be conjugated to our high quality nm black latex beads quickly and easily. The latex beads are designed for ease of use and improved handling compared with traditional latex. This has been achieved by special treatment of the particles. The conjugation is covalent, and requires less antibody than traditional passive and covalent latex conjugation.
Lateral flow immunoassays LFIAs have wide application in point-of-care testing, particularly in resource-poor settings. We also successfully applied the stacking pad system in the analysis of C-reactive protein in human serum and synovial fluid samples. These results suggest that this stacking pad LFIA can provide sensitive and on-site prognosis for detection in synovial fluid and serum samples in resource-limited settings.